Galanin and somatostatin inhibition of substance P-induced airway mucus secretion in the rat

Abstract

Substance P is present in several neurons innervating the lung. Tachykinin receptors are expressed on submucosal gland cells. Substance P is known to be a potent stimulator of airway mucus secretion. In the present study we characterized the effects of galanin and somatostatin on basal and substance P-induced mucus secretion. The stimulatory effect of substance P was concentration-dependent (100 pmol/l: 112%, 1 nmol/l: 120%, 10 nmol/l: 153%, 100 nmol/l: 223%, 1 μmol/l: 275%, 10 μmol/l: 172%) and was inhibited by galanin and somatostatin (1 μmol/l substance P: 277%; 1 μmol/l substance P+1 μmol/l somatostatin: 190%, p < 0.01; 1 μmol/l substance P+1 μmol/l galanin: 206%, p < 0.05). In the presence of lower concentrations of substance P 1 μmol/l somatostatin and 1 μmol/l galanin did not modify mucus secretion. Lower concentrations of galanin and somatostatin did not significantly change mucus secretion stimulated by 1 μmol/l substance P. Both, galanin and somatostatin at 1 μmol/l left basal airway mucus secretion unaltered. These data suggest that mucus secretion into airways is regulated by a complex network of peptidergic stimulators and inhibitors including substance P, somatostatin and galanin.