Abstract
Objective To investigate apoptosis induced by Galangin in lung cancer A549 cells.Methods Cell inhibition ratio was assayed by MTT method.Morphologic assessment of apoptosis was performed with a fluorescence microscope,and cell cycle and mitochondrial membrane potential were analyzed by flow cytometry.The expression levels of proteins were measured by Western blot.Results A549 cells were inhibited by Galangin in a dose-dependent manner.IC50 of Galangin was 30.15 mg/L.The apoptosis appeared in 24 hours after treated by Galangin.Morphologic changes including nuclear chromatin condensation fluorescence strength was observed through fluorescence microscope.Caspases were activated.The expression of Bcl-2 and Bcl-XL decreased,while the expression of p53,Bax and Bid increased.Conclusion Galangin could induce A549 cell apoptosis through mitochondria pathway.
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