Abstract
Salinity is a major environmental factor that constrains soybean yield and grain quality. Given our past observations using the salt-sensitive soybean (Glycine max [L.] Merr.) accession C08 on its early responses to salinity and salt-induced transcriptomic modifications, the aim of this study was to assess the lipid profile changes in this cultivar before and after short-term salt stress, and to explore the adaptive mechanisms underpinning lipid homeostasis. To this end, lipid profiling and proteomic analyses were performed on the leaves of soybean seedlings subjected to salt treatment for 0, 0.5, 1, and 2 h. Our results revealed that short-term salt stress caused dynamic lipid alterations resulting in recycling for both galactolipids and phospholipids. A comprehensive understanding of membrane lipid adaption following salt treatment was achieved by combining time-dependent lipidomic and proteomic data. Proteins involved in phosphoinositide synthesis and turnover were upregulated at the onset of salt treatment. Salinity-induced lipid recycling was shown to enhance jasmonic acid and phosphatidylinositol biosyntheses. Our study demonstrated that salt stress resulted in a remodeling of membrane lipid composition and an alteration in membrane lipids associated with lipid signaling and metabolism in C08 leaves.
Highlights
Soybean (Glycine max [L.] Merr.) is one of the most important legume crops used as food and feed (Hasanuzzaman et al, 2016)
As modifying in membrane lipid composition provides an effective strategy in maintaining cell membrane integrity to confront stress (Elkahoui et al, 2004), the characterization of membrane lipids following salt treatment is crucial in formulating strategies to overcome salinity
Our results suggest that the breakdown of chloroplast membrane lipids (MGDG and DGDG) would lead to the production of polyunsaturated fatty acids (PUFAs) (Boudière et al, 2014), which act as substrates for jasmonic acid (JA) biosynthesis at the onset of salt stress (Figure 4 and Supplementary Table 8)
Summary
Soybean (Glycine max [L.] Merr.) is one of the most important legume crops used as food and feed (Hasanuzzaman et al, 2016). As a major environmental constraint on crop yield, salinity triggers osmotic stress in roots followed by whole-plant ionic toxicity arising from Na+ and Cl− ion accumulation (Munns and Tester, 2008). Plants have evolved to adapt to high salt by salt-responsive signaling, balancing osmotic pressure in organic osmolyte formation, Na+ compartmentalization, enhanced scavenging of reactive oxygen species (ROS), phytohormone regulation, and cell structure modulation (Parvaiz and Satyawati, 2008; Phang et al, 2008). Salt-induced membrane lipid alterations documented in salt-sensitive varieties of Proteome and Lipidome Analyses of Salt-Treated Soybean
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
