Abstract
Gluconate dehydrogenase (GADH) biosensors have been developed for the satisfactory determination of gluconic acid in wine samples, without any pretreatment. The biosensors have been fabricated by cross-linking immobilization of GADH onto screen-printed carbon electrodes, containing the mediator tetrathiafulvalene (TTF). Chronoamperograms have been registered at +100mV vs. screen-printed Ag/AgCl electrode by successive additions of a gluconic acid solution in the concentration range from 9.0 to 131.4μM. This method shows a reproducibility of 8.1% (n=3) related to the slopes of these calibration curves and a repeatability of 3.2% (n=5). The procedure has shown an average capability of detection of 9.0μM for a probability of false positive and negative of 0.05. Wine samples have been analyzed with these biosensors, obtaining satisfactory results.
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