Gabaergic interneurons Help Bridge the Brain's Communication Gap

Abstract

Event Abstract Back to Event Gabaergic interneurons Help Bridge the Brain's Communication Gap S. Garel1, P. Gaspar2, F. Guillemot3, J. Hornung4, C. Lebrand4*, F. Mann5, M. Niquille4, B. Otsmane5, C. Parras6 and Y. Yanagawa7 1 Ecole Normale Supérieure, Inserm, U784, France 2 Institut du Fer à Moulin, INSERM U839, France 3 National Institute for Medical Research, Mill Hill, Division of Molecular Neurobiology, United Kingdom 4 University of Lausanne, Department of Cellular Biology and Morphology, Switzerland 5 Developmental Biology Institute of Marseille Luminy, Université de la Méditerranée, CNRS, UMR 6216, France 6 Laboratoire de Biologie des Interactions Neurones/Glie Inserm, U-711, France 7 Gunma University Graduate School of Medicine and SORST, Department of Genetic and Behavioral Neuroscience, Japan Subpopulations of midline glial cells have been shown to guide corpus callosum (CC) axons to the contralateral cerebral hemisphere. While neuronal cells are also present in the developing CC, their role remains obscure. In our work, we show that the CC is transiently populated by numerous glutamatergic and GABAergic neurons. These two distinct neuronal populations converge towards the midline before the callosal axons. Time-lapse and 3D imaging studies reveal that these migrating neurons settle in CC and cooperate to form barriers that funnel callosal fibres. In vivo analyses in Mash1 mutant mice which lack CC GABAergic interneurons, and rescue experiments after transplantation of wild-type CC within Mash1-/- brain slices, demonstrate that CC GABAergic interneurons are essential to guide callosal axons at the midline. Moreover, in transplantation experiments of CC neurons into heterotopic regions, both types of CC neurons facilitate the growth of callosal fibres. Finally, Semaphorin 3C (Sema3C), which is expressed by CC glutamatergic neurons, acts through neuropilin 1 (Npn-1) to elicit an attractive action on callosal axons. Taken together, our study reveals a novel role for transient neuronal populations in the development of a major cerebral commissure and brings a new perspective on the respective roles of glial and neuronal cells in this process. Conference: EMBO workshop: Gaba Signalling and Brain Networks , Amsterdam, Netherlands, 30 Jun - 2 Jul, 2010. Presentation Type: Poster Presentation Topic: Posters Citation: Garel S, Gaspar P, Guillemot F, Hornung J, Lebrand C, Mann F, Niquille M, Otsmane B, Parras C and Yanagawa Y (2010). Gabaergic interneurons Help Bridge the Brain's Communication Gap. Conference Abstract: EMBO workshop: Gaba Signalling and Brain Networks . doi: 10.3389/conf.fnins.2010.15.00014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Jun 2010; Published Online: 24 Jun 2010. * Correspondence: C. Lebrand, University of Lausanne, Department of Cellular Biology and Morphology, Lausanne, Switzerland, cecile.lebrand@unil.ch Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers S. Garel P. Gaspar F. Guillemot J. Hornung C. Lebrand F. Mann M. Niquille B. Otsmane C. Parras Y. Yanagawa Google S. Garel P. Gaspar F. Guillemot J. Hornung C. Lebrand F. Mann M. Niquille B. Otsmane C. Parras Y. Yanagawa Google Scholar S. Garel P. Gaspar F. Guillemot J. Hornung C. Lebrand F. Mann M. Niquille B. Otsmane C. Parras Y. Yanagawa PubMed S. Garel P. Gaspar F. Guillemot J. Hornung C. Lebrand F. Mann M. Niquille B. Otsmane C. Parras Y. Yanagawa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.