GABAB receptor system modulates mitragynine-induced conditioned place preference in rats

Abstract

Event Abstract Back to Event GABAB receptor system modulates mitragynine-induced conditioned place preference in rats Nurul H. Mohammad Yusoff1*, Sharif M. Mansor1, Navaratnam Visweswaran1, Christian P. Muller2 and Zurina Hassan1* 1 Universiti Sains Malaysia, Centre for Drug Research, Malaysia 2 Friedrich-Alexander-University Erlangen-Nuremberg, Department of Psychiatry and Psychotherapy, Germany Many drugs of abuse produce rewarding effects, as evidenced by their ability to induce conditioned place preference (CPP). Mitragynine, the major active alkaloid of Mitragyna speciosa (Kratom) plant, has been reported to possess this response in animal model. Considering the role of GABAB receptor subtypes in mediating the rewarding properties of various classes of addictive drugs, the present study aims to examine the involvement of GABAB receptor system on acquisition and expression of mitragynine-induced CPP. An unbiased 3-chamber CPP paradigm was employed, which consisted of 3 phases; pre-conditioning (2 days), conditioning (8 days), and CPP test. Pre-conditioning test was performed to assess initial individual preference for each CPP chamber. Groups of rats were conditioned with mitragynine (10 mg/kg), combination of mitragynine and baclofen (1.25, 2.5 and 5.0 mg/kg) or vehicle paired with a distinctive chamber. On alternating days, rats were given vehicle and paired with another distinctive chamber. After 8 conditioning sessions, a preference (CPP) test was conducted whereby rats had full access to the entire apparatus under the influence of either baclofen (1.25, 2.5 and 5.0 mg/kg) or saline. Mitragynine-conditioned animals showed CPP as demonstrated by an increased amount of time spent in the drug-associated chamber during the CPP test relative to pre-conditioning test. The administration of baclofen (2.5 and 5.0 mg/kg) 30 min prior to mitragynine injection inhibited the acquisition of mitragynine-induced CPP. In addition, the administration of baclofen (2.5 and 5.0 mg/kg) to the mitragynine-conditioned animals 30 min prior to the CPP test blocked the expression of CPP responses. These findings showed that activation of GABAB receptors by baclofen suppressed the acquisition and expression of CPP effects produced by mitragynine. Mitragynine-induced CPP effects are dependent on the activity of GABAB receptor system during acquisition and expression phases. Acknowledgements Financial support was received from Higher Education Centre of Excellence (HiCoE) special funding (304/CDADAH/650527/K134) and FRGS (203/CDADAH/6711469) Keywords: Addiction, GABA, conditioned place preference, in vivo, rewarding effect Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: O03: Postgraduate Travel Awardees Oral Session 3 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Mohammad Yusoff NH, Mansor SM, Visweswaran N, Muller CP and Hassan Z (2016). GABAB receptor system modulates mitragynine-induced conditioned place preference in rats. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00090 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Ms. Nurul H Mohammad Yusoff, Universiti Sains Malaysia, Centre for Drug Research, Minden, Penang, Malaysia, hess_myz@yahoo.com Dr. Zurina Hassan, Universiti Sains Malaysia, Centre for Drug Research, Minden, Penang, Malaysia, zurina_hassan@usm.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nurul H Mohammad Yusoff Sharif M Mansor Navaratnam Visweswaran Christian P Muller Zurina Hassan Google Nurul H Mohammad Yusoff Sharif M Mansor Navaratnam Visweswaran Christian P Muller Zurina Hassan Google Scholar Nurul H Mohammad Yusoff Sharif M Mansor Navaratnam Visweswaran Christian P Muller Zurina Hassan PubMed Nurul H Mohammad Yusoff Sharif M Mansor Navaratnam Visweswaran Christian P Muller Zurina Hassan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.