GABA C receptor mediated inhibition in acutely isolated neurons of the rat dorsal lateral geniculate nucleus

Abstract

In the dorsal lateral geniculate nucleus (dLGN), GABA C receptors seems to be specifically expressed by local GABAergic interneurons. Although the presence of GABA C receptors has been demonstrated, a quantitative estimation of their contribution to inhibition in dLGN is lacking. Because the amount of inhibition mediated by these receptors might reflect their functional importance we performed whole-cell patch clamp recordings from dLGN cells acutely dissociated from brain slices. We focally applied the GABA receptor agonist muscimol and quantified effects mediated through either GABA C or GABA A receptors. Because their basic dendritic morphology was preserved, we tried to morphologically differentiate between thalamocortical cells and local interneurons. In the majority of multipolar cells, representing thalamocortical projection neurons, the specific GABA A receptor antagonist bicuculline completely blocked muscimol induced currents. In contrast, in most of the bipolar cells, representing interneurons, bicuculline blocked only 70–80% of the muscimol induced currents. The remaining currents were blocked by co-application of TPMPA, a specific GABA C receptor antagonist, or picrotoxin, an unspecific GABA A and GABA C receptor blocker. The latter neurons were also sensitive to the selective GABA C receptor agonist cis-aminocrotonic acid. These results indicate that in those dLGN neurons that express GABA C receptors, these receptors contribute considerably to GABAergic inhibitory inputs.