GABA B receptor activation inhibits dopamine D1 receptor-mediated facilitation of [ 3H]GABA release in substantia nigra pars reticulata

Abstract

GABA B receptors inhibit and dopamine D1 receptors stimulate the release of GABA from striatal terminals in the pars reticulata of the substantia nigra. Here we have studied the interaction between both classes of receptors by exploring the effect of GABA B receptors upon the stimulation of depolarization-induced [ 3H]GABA release induced by the activation of D1 receptors in slices of the pars reticulata of the rat substantia nigra. The activation of GABA B receptors with baclofen (100 μM) inhibited by 48 ± 8% the evoked [ 3H]GABA release in normal slices but did not modify the release in slices from reserpine-treated rats, indicating that the inhibition was dependent on endogenous dopamine. The inhibitory effect of baclofen was also abolished by the D1 receptor antagonist SCH 23390 (1 μM), indicating a D1 receptor-dependence of the baclofen inhibition. Baclofen dose-dependently inhibited (IC 50 = 3.6 μM) the stimulation of release induced by the D1 agonist SKF 38393 (1 μM). Baclofen also blocked the stimulation of release induced by forskolin but not that induced by 8-Br-cAMP, indicating that the inhibitory effect was exerted before cAMP synthesis. N-ethylmaleimide (NEM), a selective inactivator of PTX-sensitive G-proteins, abolished the baclofen inhibition of the SKF 38393-induced stimulation of the release without affecting the stimulation induced by the D1 agonist, suggesting that the baclofen effect was mediated by Gα i/o proteins. These results might have relevance in the control motor disorders associated with D1 receptor supersensitivity.