GaAlAs laser pretreatment enhances the function of ADSCs in photoaging of the skin in rats

Abstract

Objective To investigate the effect of adipose-derived stem cells (ADSCs) on photoaging skin after laser pretreatment with GaAlAs. Methods ADSCs were isolated from healthy wistar rats, ADSCs were isolated and cultured to establish an adipose-derived stem cell culture system. ADSCs were pretreated with GaAlAs laser at a wavelength of 650 nm 4 J/cm2. A rat model of photoaging aging was established. Different doses of ADSCs and low energy laser ADSCs were pretreated with ADSCs for the treatment of photoaging skin, and the morphological changes of epidermis and dermis were observed before and after treatment with low energy laser pretreatment. Results When the concentration of ADSCs was 103/100 μl, there was no significant difference in epidermal thickness and dermal thickness between ADSCs treated group and GaAlAs pretreatment group (P>0.05). The thickness of epidermis in the GaAlAs pretreatment group was significantly lower than that in the ADSCs group (P<0.05) at 104/100 μl. When the concentration of ADSCs was 5×104/100 μl, the epidermal thickness of the GaAlAs pretreatment group decreased significantly and the thickness of the dermis increased significantly, which was significantly different from that of the ADSCs group (P<0.05). Conclusions GaAlAs laser pretreatment can enhance ADSCs anti-skin photoaging ability. Key words: Lasers; Multipotent stem cells; Cellular senescence; GaAlAs; Adipose-derived stem cells