G350 of Escherichiacoli RNase P RNA contributes to Mg2+ binding near the active site of the enzyme

Abstract

G350 of Escherichiacoli RNase P RNA is a highly conserved residue among all bacteria and lies near the known magnesium binding site for the RNase P ribozyme, helix P4. Mutations at G350 have a dramatic effect on substrate cleavage activity for both RNA alone and holoenzyme; the G350C mutation has the most severe phenotype. The G350C mutation also inhibits growth of cells that express the mutant RNA in vivo under conditions of magnesium starvation. The results suggest that G350 contributes to Mg2+ binding at helix P4 of RNase P RNA.