Abstract
One of critical issues of bone tissue engineering is the establishment of technique to effectively promote the calcification of regenerative bone in culture. We have found that a random pulse tram electromagnetic fields (EMF) can promote more the calcification of osteoblasts than a periodic pulse train EMF in vitro. However, the mechanism behind this result is unknown. Cellular proliferation and alkaline phosphatase (ALP) activity are two of factors related to osteoblastic calcification. The purpose of this study is to investigate the effect of random pulse train EMF on the proliferation and ALP activity of osteoblasts in vitro MC3T3-E1 osteoblastic cells were seeded on a φ 35-mm culture dish at the density of 100 cells/mm^2. The cells on the central area within a radius of 9 mm were exposed to EMF for three minutes per day. The effect of random pulse train (RdPT) EMF was compared with those of periodic pulse tram (PrPT) EMF, continuous constant (CC) EMF, and no EMF stimulation control. The detection of ALP activity was conducted by using the lead citrate method on the culture dish. The cells proliferation did not show any difference among all the groups. On the other hand, in ALP activity, RdPT group showed the highest values among all the groups. These results suggest that the osteogenic effect of random pulse train EMF depends on the differentiation stage of osteoblasts, giving us useful information about the mechanism.
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