G protein beta 3(GNβ3) C825T polymorphism and irritable bowel syndrome susceptibility: an updated meta-analysis based on eleven case-control studies.

Dongbo Jiang,Huayan Chen,Xiaoli Ma,Yan Wang,Ting Li,Weiming Cai,Dong Huang,Tangming Guan

doi:10.18632/oncotarget.23449

Abstract

Several studies have reported an association between GNβ3 C825T polymorphism and irritable bowel syndrome (IBS). However, the results remain inconclusive and controversial, particularly for the data derived from different ethnicities and IBS subtypes. Therefore, we performed an updated meta-analysis to evaluate this association. All eligible case-control studies that met the search criteria were retrieved from multiple databases, and eleven case-control studies were included for detailed evaluation. The pooled odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated to assess the strengths of the association between GNβ3 C825T polymorphism and susceptibility to IBS and its subtypes. Our meta-analysis found no significantly associations of GNβ3 C825T polymorphism with IBS risk in all populations. Whereas the C allele was demonstrated to be a decreased risk factor for constipation predominant IBS (IBS-C) in allele model. Additionally, the CC genotype was found to be associated with increased diarrhea predominant IBS (IBS-D) risk in recessive model. Subgroup analysis by ethnicity revealed that these associations held true for the Asian subpopulation. In conclusion, this meta-analysis suggests the C allele of GNβ3 C825T might be associated with a decreased risk of IBS-C, and the CC genotype of GNβ3 might be associated with increased IBS-D risk.

Highlights

Irritable bowel syndrome (IBS) is the most prevalent gastrointestinal disorder characterized by abdominal discomfort, pain, and altered defecation patterns; it may considerably reduce patients’ quality of life and work productivity, which affects more than 7 percent of people all around the world [1, 2]
Our meta-analysis found no significantly associations of G-protein β3 subunit gene (GNβ3) C825T polymorphism with irritable bowel syndrome (IBS) risk in all populations
This meta-analysis suggests the C allele of GNβ3 C825T might be associated with a decreased risk of IBS patients can experience constipation (IBS-C), and the CC genotype of GNβ3 might be associated with increased diarrhea predominant IBS (IBS-D) risk

Summary

INTRODUCTION

Irritable bowel syndrome (IBS) is the most prevalent gastrointestinal disorder characterized by abdominal discomfort, pain, and altered defecation patterns; it may considerably reduce patients’ quality of life and work productivity, which affects more than 7 percent of people all around the world [1, 2]. The C825T polymorphism of GNβ3 (rs5443) is associated with www.impactjournals.com/oncotarget alternative splicing of the gene and its protein activity, and an increased intracellular signal transduction compared with unmodified Gβ3 protein [15, 16]. This single nucleotide polymorphism (SNP) has been reported to be associated with depression [17], Alzheimer’s disease (AD) [18], hypertension [19], obesity [20], Insulin-mediated venodilation [21], Vasculogenic erectile dysfunction (VED) [22], and functional dyspepsia [23].

RESULTS

Genotyping Method

DISCUSSION

MATERIALS AND METHODS