G.P.130 The effect of water-soluble fullerene in muscle regeneration process of experimental murine skeletal muscle

Abstract

<h2>Abstract</h2> Muscular dystrophy is a hereditary disease, which causes severe muscle weakness and atrophy clinically, and skeletal muscle degeneration and necrosis in pathology. In recent years, the importance of involvement of oxidative stress by acute and chronic inflammation was revealed in the process of muscle degeneration. Therefore, reducing oxidative stress is considered as one of the new treatment strategy in muscular dystrophy. Fullerene is an allotrope of carbon the same as diamond, and has been reported that it can detoxify and absorb free radical. To evaluate the effectiveness of water-soluble fullerene, we chronologically evaluate the histology in mice tibial muscles during a cycle of regeneration induced by cardiotoxin injection. Tibial muscles of C57BL10 mice (4–5weeks old) were injected with cardiotoxin with fullerene or without fullerene. The injected muscles were removed and stained for H&E on 1, 3, 5, 7, 14, and 28days after the injection. Supernatants of muscle extract were prepared and western-blotting was performed by Laemmli methods to evaluate expression of muscle proteins, such as dystrophin, desmin, and nNOS. Average diameter of regenerate muscles 28days after injection, cardiotoxin group and co-administered fullerene group were 19.8±7.6μm and 27.0±7.6μm, respectively. Muscle protein expression in co-administered fullerene group, dystrophin, desmin, and nNOS was observed in the earlier stage by Western blot than those of cardiotoxin group. During the experiment, no significant and critical change was observed.We revealed that Fullerene has a function to promote the regeneration of skeletal muscle in this experiment. We believe fullerene and can be applied in the treatment of muscular dystrophy.