Fuzi polysaccharide isolated from Aconitum carmichaeli protects against liquid nitrogen cryopreservation-induced damage in rat abdominal aorta by enhancing autophagy

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ObjectiveTo investigate the potential protective mechanisms of aconite polysaccharide (FZPS-1) during cryopreservation, with a particular emphasis on morphological changes in autophagy in rat abdominal aorta. MethodsThirty-six male SD rats were divided into the control group, the cryopreserved model group, and the FZPS-1 intervention group treated with different concentrations of FZPS-1. The structural changes of the abdominal aortic wall were assessed via Masson staining, while cytolysosomes were identified using transmission electron microscopy (TEM). The expression of Beclin-1, LC3-II, and P62 was detected by immunohistochemistry (IHC) and western blot separately. Bcl-2 and Bax mRNA expression was measured by RT-qPCR. ResultsCompared with the control group, the abdominal aortic wall in the model group was severely damaged. Contrarily, FZPS-1 10 mg/mL and 20 mg/mL groups had relatively normal structure of the blood vessel wall, higher cytolysosome counts, and increased Beclin-1 and LC3-II expression compared with the model group (all P<0.05); P62 expression also increased in the FZPS-1 20 mg/mL group (P<0.05). Compared with the control group, the mRNA expression of Bcl-2 in the cryopreservation model group was reduced (P<0.05), while Bax was increased (P<0.05). Compared with the cryopreservation model group, the mRNA expression of Bcl-2 was upregulated, while Bax was downregulated in the FPS 10 mg/L group (P<0.05). ConclusionDuring liquid nitrogen cryopreservation, autophagy is inhibited in the rat abdominal aorta, and the blood vessel wall structure is damaged. FZPS-1, as a cryoprotectant, can enhance autophagy and mitigate blood vessel wall damage in the rat abdominal aorta.