Future issues in forensic computing and an introduction to ENSFI

Abstract

The aim was investigate resveratrol, grape juice, or red wine effects on FNDC5 and UCP2 gene expression on muscle and adipose tissue of Wistar rats receiving a high-fat diet.Five groups of female Wistar rats formed with five animals in each (Control Group-CG, High-Fat Group-HFG-20%, Grape Juice Group-GJG; Red Wine Group-RWG and Resveratrol Group-RG) were treated for 2 months. At the end, adipose and muscle tissues were collected, FNDC5 and UCP2 mRNA expressions were obtained by using real-time polymerase chain reaction. Irisin levels were assessed through ELISA. For comparison: two way ANOVA, using Duncan as post-test.FNDC5 mRNA expression was increased in RG and GJG muscle (p < 0.001) and reduced in adipose tissue of all experimental groups (p < 0.001). The UCP2 mRNA expression was increased in muscle of HFG, RG and RWG (p < 0.01), compared with CG. Furthermore, increased expression of UCP2 mRNA in GJG adipose tissue (p < 0.05) was also observed when compared with the control group (CG). No significant differences were found concerning plasma irisin level in all animal groups.Resveratrol and grape juice were able to increase muscle tissue FNDC5 gene expression, and high-fat diet, red wine and resveratrol, increased UCP2 gene expression in this tissue. Grape juice was capable of increasing adipose tissue UCP2 gene expression. High-fat diet, isolated or associated to beverages rich in polyphenols, have decreased FNDC5 gene expression in adipose tissue. Nevertheless, the interventions did not affect irisin levels.