Abstract
Isolated livers from fed rats were perfused with a medium containing glucose labeled uniformly with 14C and specifically with 3H. There was considerable formation of glucose from endogenous sources but simultaneously uptake of about half of the 14C in glucose. After 2 hours the 3H 14C ratios in perfusate glucose decreased by 55–60% with (2- 3H, U- 14C), 40–50% with (5- 3H, U- 14C), 25–30% with (3- 3H or 4- 3H, U- 14C) and by 10–15% with (6- 3H, U- 14C) glucose. Qualitatively comparable patterns were obtained with rat hepatocytes. These results demonstrate recycling of carbon between glucose and pyruvate. Superimposed upon this there is an extensive futile cycle between glucose and glucose 6-P. There is also futile cycling between fructose 6-P and fructose 1,6 P 2 and to a small extent between phosphoenol pyruvate and pyruvate.
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