Fusion Pore Expansion and Contraction during Catecholamine Release from Endocrine Cells

Abstract

Amperometry recording reveals the exocytosis of catecholamine from individual vesicles as a sequential process, typically beginning slowly with a prespike foot, accelerating sharply to initiate a spike, reaching a peak, and then decaying. This complex sequence reflects the interplay between diffusion, flux through a fusion pore, and possibly dissociation from a vesicle’s dense core. In an effort to evaluate the impacts of these factors, a model was developed that combines diffusion with flux through a static pore. This model accurately recapitulated the rapid phase of a spike but generated relations between spike shape parameters that differed from the relations observed experimentally. To explore the possible role of fusion pore dynamics, a transformation of amperometry current was introduced that yields fusion pore permeability divided by vesicle volume (g/V). Applying this transform to individual fusion events yielded a highly characteristic time course. g/V initially tracks the current, increasing ∼15-fold from the prespike foot to the spike peak. After the peak, g/V unexpectedly declines and settles into a plateau that indicates the presence of a stable postspike pore. g/V of the postspike pore varies greatly between events and has an average that is ∼3.5-fold below the peak value and ∼4.5-fold above the prespike value. The postspike pore persists and is stable for tens of milliseconds, as long as catecholamine flux can be detected. Applying the g/V transform to rare events with two peaks revealed a stepwise increase in g/V during the second peak. The g/V transform offers an interpretation of amperometric current in terms of fusion pore dynamics and provides a, to our knowledge, new frameworkfor analyzing the actions of proteins that alter spike shape. The stable postspike pore follows from predictions of lipid bilayer elasticity and offers an explanation for previous reports of prolonged hormone retention within fusing vesicles.