Abstract
Hybridization of somatic cells has been suggested as a possible technique to overcome the cross-incompatibility of unrelated plant species. Somatic hybrids might be of great value for crop improvement (Schenk and Hildebrandt 1968). Hybridization of somatic plant cells requires the following steps: a) Removal of the cell walls to form protoplasts. b) Induction of close membrane contact at the molecular level and reversible membrane breakdown for fusion between two independent protoplasts (Poste and Allison 1973; Lucy 1978; Zimmermann and Vienken 1982). c) Identification and selection of the somatic hybrids. d) Culture of the hybrid protoplasts. e) Regeneration of plants from the hybrids. KeywordsSomatic HybridProtoplast FusionPlant ProtoplastSodium Nitrate SolutionGlyceryl MonooleateThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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