Abstract
An ovoid to rod shaped, white to brown pigmented, facultative anaerobic, mesophilic, non-phototrophic, Gram-staining-negative, non-motile, multiply by binary fission designated strain KVB23T, which was isolated from root of rice plant, near Ilsan, South Korea, was investigated for its taxonomic position by polyphasic approach. Optimal growth was found to occur at 30˚C, at pH 6.5 and in the absence of NaCl on R2A. Phylogenetic analysis based on the 16S rRNA gene sequence of strain KVB23T revealed that it formed a distinct lineage, as a separate deep branch within the family Rhodobacteriaceae, with < 96.5% sequence similarity to representatives of the genera Rhodobacter, Xinfangfangia, Tabrizicola, Falsirhodobacter, Haematobacter, Paenirhodobacter, Pseudorhodobacter and Pararhodobacter. Based in 16S rRNA sequences strain KVB23T was most closely related to Tabrizicola fusiformis KCTC 62105T (96.5%) and Rhodobacter thermarum KCTC 52712T (96.2%). The draft genome of strain KVB23T was 3.80bp long with a DNA G + C content of 63.1%. Genome of strain KVB23T harboured gene clusters for tryptophan and cobalamin biosynthesis. The strain contained Q-10 as the sole respiratory quinone. The predominant fatty acids were found to consist of C16:0, C18:0 and summed feature 8 (comprising C18:1 ω7c and / or C18:1 ω6). The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, seven unidentified phosphoglycolipids, two unidentified aminophosphoglycolipid, one unidentified glycolipid and four unidentified lipids. Phosphate-solubilizing bacteria have the ability to dissolve insoluble phosphates and enhance the soil fertility. Strain KVB23T can solubilize calcium phosphate tribasic. Phosphate solubilizing and tryptophan biosynthesis property of strain KVB23T could be a possible factor for the increase in growth of rice plant. Differential phenotypic, chemotaxonomic and genotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain KVB23T was found to represent a novel genus in the Rhodobacteriaceae family, for which the name Fuscibacter oryzae gen. nov., sp. nov. is proposed, with the type strain KVB23T(= KACC 21711T = NBRC 114716T).
Highlights
The Rhodobacteriaceae familywas first established by Garrity et al (2005) as a sole member of the order Rhodobacterales within the class Alphaproteobacteria andphylum Proteobacteria.Rhodobacteriaceaeis one of the most widely distributed bacterial lineages in marine habitats such as seawater, sediments, marine snails, marine sponges and marine phytoplankton etc
There are approximately 220 recognized genera in the family at the time of writing.In the course of screening the bacterial diversity in the roots of rice plants near Dongguk university, Ilsan, South Korea, strain KVB23T was isolated from a paddy field in the, Republic of Korea.Phosphate solubilizing bacteria play important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments (Das et al 2007)
We introduce a novel non-phototrophic and phosphate-solubilizingbacterium isolated from the roots of rice plants that belongs to a new genus associated with the family Rhodobacteriaceae
Summary
The Rhodobacteriaceae familywas first established by Garrity et al (2005) as a sole member of the order Rhodobacterales within the class Alphaproteobacteria andphylum Proteobacteria.Rhodobacteriaceaeis one of the most widely distributed bacterial lineages in marine habitats such as seawater, sediments, marine snails, marine sponges and marine phytoplankton etc. There are approximately 220 recognized genera in the family at the time of writing (https://lpsn.dsmz.de/family/rhodobacteraceae).In the course of screening the bacterial diversity in the roots of rice plants near Dongguk university, Ilsan, South Korea, strain KVB23T was isolated from a paddy field in the, Republic of Korea.Phosphate solubilizing bacteria play important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments (Das et al 2007).
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