Fusarium oxysporum as causal agent of tomato wilt and fruit rot

Abstract

Tomatoes are parasitized by a number of pathogens, including Fusarium oxysporum f. sp. lycopersici, causal agent of fusarium wilt. Fresh vegetable fruits can be contaminated with various fungi that produce mycotoxins, which is an important issue for human health. The objective of this paper was to isolate, determine, and identify causal organisms of tomato wilt and fruit rot, based on the pathogens morphological and molecular characteristics. Samples of diseased plants showing symptoms of tomato wilt were collected from different localities in the production region of Vojvodina. Fruits with symptoms of fusarium rot were collected from storage and warehouses. The isolation and morphological determination of the fungus were performed on PDA and Czapek?s nutrient media. Isolates from diseased plants growing in field, designated as TFW1-TFW12 and seven isolates from diseased tomato fruits (TFM1-TFM7) were chosen for further investigation. For identification of the fungal isolates, the polymerase chain reaction (PCR) was also used. The EF1/EF2 primer pair was used for molecular identification of Fusarium sp. Nine analyzed samples were found to contain DNA fragments 700 bp in size.