Abstract
RNA synthesized in vitro by enzyme-template complexes isolated from λ lysogens at early or late times following induction has been shown by competition-hybridization procedures to resemble messenger RNA transcribed in vivo at the same stage of viral development, and to differ from RNA made in vitro by purified Escherichia coli RNA polymerase. It is demonstrated here that RNA synthesis by such complexes involves elongation of chains which have been started in vivo, rather than initiation of new RNA chains in vitro.
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