Abstract
Our previous finding of an association between herpes simplex virus type 1 (HSV-1) and African green monkey kidney (BSC-1) DNAs in productively infected cells has been extended by investigating the influence of the time course, temperature, and multiplicity of infection on the extent of this association. The interaction of host and viral DNAs was not only dependent on the concentration of intracellular viral DNA, but also upon the particular conditions of infection. An examination of the time course of infection revealed that the amount of free viral DNA per cell reached a maximum well before the amount of viral-host-associated DNA did so, while an examination of the effect of temperature revealed a threefold variation in the amount of viral-host-associated DNA over a temperature range (33.5–39.5°) in which the amount of free viral DNA was essentially constant. The quantity of HSV-1 DNA associated with host-cell DNA ranges from about 100 to 1500 genome equivalents per cell, depending on the conditions of infection, and it appears that between 5 and 40% of the association is alkali stable.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
