Abstract
ABSTRACT In this paper evidence is presented that a 27S Optical Density (O. D.) peak is absent and a 32S O. D. peak is regularly present on analysis of the soluble thyroid proteins of the goitrous rat. This occurs irrespective of the goitrogenic stimulus and a normal O. D. pattern can be restored by reversal of the goitrous process by thyroxine. In rats rendered goitrous by a low iodine diet or KClO4 treatment, iodine supplementation also removed the 32S O. D. peak and resulted in reformation of the 27S peak. In rats treated by antithyroid drugs of the thiocarbamide group loss of the 32S O. D. peak was not associated with recovery of a 27S peak. This may be related to differences in potency of the various antithyroid drugs. 125I and 3H-leucine were not incorporated into the 32S O. D. peak material of the rat thyroid in vivo or into the 32S O. D. peak material of sheep thyroid slices in vitro. The view that this 32S O. D. peak was not iodoprotein nature was confirmed by showing that this peak had a maximal absorbance at 260 nm favouring the hypothesis that it was composed of nucleic acid.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
