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Abstract
SummaryA method is described for the preparation of follicle-stimulating preparations by tryptic digestion and the removal of a fraction which caused reactions in the human being at the site of injection.Preparations made by the tryptic digestion method before and after it was changed to remove the local reacting factor were assayed both in normal and hypophysectomized rats. By macroscopic observation the ovaries from both kinds of rats appeared to contain only follicles but microscopic examination showed that a few contained lutein tissue indicating that not all the preparations were entirely devoid of luteinizing activity.
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