Abstract
IN A PREVIOUS paper (Lacey, 1941) the method of insulin assay used in the Insulin Committee Laboratory, University of Toronto, was described. This procedure, with minor modifications, was adopted in 1942 as the official method by the United States Pharmacopoeial Convention (Pharmacopoeia of the United States, 1942). The biological and mathematical modifications approved by the Convention have been incorporated into the method in regular use in the Insulin Committee Laboratory, University of Toronto. The method has had extensive trial in this laboratory, in the laboratories of insulin producers in the United States and of the United States Food and Drug Administration. That the method is of general application is borne out by the results of unpublished collaborative studies in evaluation of potency of the most recent insulin reference standard for the Insulin Committee and the United States Pharmacopoeia.
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