Abstract
A simplified method was developed for preparing rabbit red cells coated only with human C3d by the alternative pathway; these cells were injected into the donor rabbit to prepare an anti-C3d serum. Monkey red cells were sucessfully coated with human C4d, using a low-ionic-strength method, and these cells were injected into the donor monkey to prepare anti-human C4d. The reactions of these reagents were compared with those of antisera raised to complement-coated red cells by previously described methods and with those of antisera obtained by immunizing rabbits with purified soluble human C3d.
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