Abstract
SUMMARY: Intraspecific transformation in three strains of Pseudomonas fluorescens was observed with respect to gelatin liquefaction and chlortetracycline resistance. In interspecific transformation experiments involving 23 pairs of strains and four markers, two yielded positive results: a strain of P. fluorescens acquired the property of gelatin liquefaction when transformed with P. aeruginosa DNA, and a strain of fish Pseudomonas, incapable of growing at 37 °C, did so when transformed with DNA from another strain of P. aeruginosa. The guanine-cytosine content of the DNA of the strains investigated ranged between 61.0 and 64.4 mol%. The transforming ability of DNA was destroyed by DNase. The divalent ions Mg2+, Ca2+ and Ba2+ improved transformation frequency several fold. Actinomycin D inhibited transformation.
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