Further morphological (freeze-fracture) evidence for an impulse generating function of Mauthner axon collaterals in the tench ( Tinca tinca L.) spinal cord

Abstract

The freeze-etching technique was applied to the tench spinal cord. Replicas of Mauthner axons revealed that the non-junctional axolemma at the tip of axon collaterals alone exhibits intramembranous E-face particles whose size, distribution and overall density are comparable with those at Ranvier nodes. Since typical Ranvier nodes are lacking along the stem of the heavily myelinated Mauthner giant axon, this finding contributes further evidence to our earlier observations suggesting that the spiny Mauthner axon collaterals represent true nodal equivalents and most likely the long-sought substrate of impulse propagation.