Abstract
Can simian virus 40 (SV40) be used to detect mutagenic DNA repair in cultured mammalian cells? The published evidence from different laboratories are in direct conflict. In order to decide between the conflicting evidence, we conducted experiments in two separate laboratories using experimental protocols similar to those previously used to investigate mutagenic repair with viral probes. Mutagenesis in SV40 virus stocks obtained by infecting ultraviolet (UV)-irradiated or unirradiated CV-1 monkey kidney cells with UV-irradiated or unirradiated temperature-sensitive SV40 mutant tsB201 was investigated. The frequency of reversion of the ts mutant to phenotypically wild-type virus was determined by assaying the virus stocks at permissive (33°) and non-permissive (39°) temperatures. These data show that (a) the reversion frequency for unirradiated virus propagated in irradiated cells was more than that in unirradiated cells; (b) irradiated virus gave more reversion than unirradiated virus in unirradiated and irradiated cells; and (c) irradiated virus had a lower reversion frequency in irradiated cells than in unirradiated cells. Reactivation experiments carried out in parallel with the mutagenesis showed enhanced reactivation in UV-irradiated SV40 in UV-irradiated CV-1 cells. We conclude that enhanced reactivation of UV-irradiated SV40 was not mutagenic in monkey kidney cells.
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