Abstract
A stretch of the 23S rDNA from Clostridium botulinum strains that produce different types of neurotoxins was sequenced and three rDNA oligonucleotide probes designed. They were labelled non-radioactively with DIG-ddUTP and tested for specificity by dot blot hybridization against rRNA of 83 strains of clostridia and other Gram-positive organisms. The probes discriminated between groups of C. botulinum strains defined by their toxin types thus supporting previous results on DNA-DNA and DNA-rRNA similarity studies and phenotypic characterization: (i) proteolytic C. botulinum strains with toxins of types A, B and F, as well as the closely related strains of C. sporogenes and C. putrificum and a strain of C. barati that produces a botulinum toxin of type F; (ii) non-proteolytic C. botulinum strains with toxins B and E; (ii) C. botulinum with toxin type D, and (iii) C. argentinense (formerly C. botulinum ) with botulinum toxin type G, as well as C. subterminale . These probes extend the spectrum of C. botulinum probes published recently for non-proteolytic C. botulinum types B, E and F ( Campbell et al., 1993).
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