Abstract
This paper describes further characterization of the 170–180‐kDa glycoprotein (P‐glycoprotein) recognized by the monoclonal antibody MRK 16 in the human adrenal. By electron microscopy, P‐glycoprotein was observed in the adrenal cell membranes. However, MRK 16‐defined P‐glycoprotein was not found in cow, pig, horse, monkey or rabbit adrenal, indicating that MRK 16 recognizes the non‐homologous part of P‐glycoprotein of various species. Eleven out of 16 adrenal tumors including 4 cases of primary aldosteronism and 7 cases of Cushing syndrome were intensely stained with MRK 16, whereas pheochromocytoma, non‐functioning adrenocortical adenoma with no associated increase of serum adrenal‐derived hormones and myolipoma of the adrenal were not. Finally, P‐glycoprotein‐MRK 16‐protein A‐Sepharose complex derived from human adrenal possessed marked ATPase activity. Taken together, these data suggest that P‐glycoprotein may play a physiological role in the human adrenal.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
