Abstract
AbstractThe enzymes responsible for nitrate reduction in Chlamydomonas reinhardii, namely NADH‐nitrate reductase and ferredoxin‐nitrite reductase, have been further characterized. The first activity of the nitrate reducing complex, NADH‐diaphorase, is protected by FAD against thermic inactivation. This fact suggests an important structural and functional role for this nucleotide in the first moiety of the nitrate reductase complex. The effect of p‐hydroxymercuribenzoate on the diaphorase activity and the protection by NADH against its inactivation indicate that some—SH groups participate in the electron transfer mediated by diaphorase.Radioactive labelling of nitrate reductase with 99Mo and 185W as well as competition experiments between Mo and W indicate that molybdenum is an essential component of terminal nitrate reductase activity. Iron seems to participate in the redox processes mediated by both nitrate and nitrite reductases as suggested by experiments performed at physiological level. Finally a tentative mechanism for the whole process of nitrate assimilation in Chlamydomonas is proposed.
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