Abstract
To investigate the presence of furosine in commercial samples of jams and fruit-based infant foods a simple method by ion-pair reversed-phase liquid chromatography is described. The yield of furosine during the hydrolysis with hydrochloric acid was optimized. The reproducibility in the repeatability and recovery of the method, expressed in relative standard deviation percentages, proved to be in the ranges of 4.1-8.3% and 1.0-4.4%, respectively. The recovery percentages of furosine varied between 86.7 and 95.3%. The obtained results support the suitability of the method. Furosine was detected in all studied samples. Although a high variability in the content of furosine was noticed, in general terms, the lowest levels of furosine were observed in samples of fruit-based infant foods and the highest were observed in jams of more than 60% sugar. These results could be due to different heat treatment, storage conditions, and/or differences in the values of water activity (a(w)) and amounts of sugar. The results obtained in the present paper point out the usefulness of furosine as an indicator of Maillard reaction for jams and fruit-based infant foods.
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