Abstract
The compound 1,4‐dimethylnaphthalene (DMN) was originally isolated from dormant potatoes and is currently being used as a commercial sprout inhibitor. In order to determine how the microbiome of potatoes is affected by the compound, two fungal species were grown and quantified under control and DMN conditions: Pythium ultimum and Aspergillus niger. These two fungal species are commonly found on the surface of potatoes and can result, respectively, in Pythium leak and black rot on onions. Fungal cultures were obtained from ATCC (P. ultimum: 58811, A. niger: 16888) and grown on potato dextrose agar (PDA). Mycelial cultures were grown in 9.5 Liter BBL GasPak chambers, exposed to either mineral oil (control) or DMN for two days. The mycelial mats exhibited reduced growth under DMN conditions in comparison to controls. DMN treatment of mature A. niger cultures also prevented sporulation. This indicates that DMN displays fungistatic properties but does not function as a fungicide towards P. ultimum and A. niger. In order to determine how DMN affects the gene expression of these fungi, RNA sequences will be collected and gene expression will be observed between control and DMN treatments.Support or Funding InformationSupported in part by a grant from the 1,4‐Group of Meridian, ID and the Behrend College Undergraduate Research FundThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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