Fungistatic effect of agrochemical and pharmaceutical fungicides on non-target aquatic decomposers does not translate into decreased fungi- or invertebrate-mediated decomposition

Abstract

Leaf litter decomposition is a key ecological process in freshwater ecosystems. Fungi, particularly aquatic hyphomycetes, play a major role in organic matter turnover and constitute a pivotal node in detrital food webs. The extensive use of antifungal formulations, which include agrochemicals and pharmaceuticals, is a threat to biodiversity and may affect non-target microbial and invertebrate decomposer communities. Using a laboratory approach, we assessed the effects of tebuconazole (agrochemical), clotrimazole and terbinafine (pharmaceuticals) on aquatic communities and on the decomposition of plant litter. Alder leaves were colonized by natural microbiota in a clean stream, and then exposed in microcosms to 8 concentrations of each fungicide (10 to 1280 μg L−1). Fungicides led to shifts in species dominance in all tested concentrations, but no effects on leaf decomposition were observed. In addition, tebuconazole and clotrimazole strongly reduced fungal biomass and reproduction, whilst terbinafine stimulated fungal reproduction at lower concentrations but had no measurable effects on fungal biomass. Subsequently, the indirect effects of the fungicides were assessed on the next trophic level (detritivore invertebrates), by evaluating leaf consumption by a specialist (Allogamus sp.) and a generalist (Chironomus riparius) species, when feeding on fungicide-preconditioned leaves. The feeding activity of C. riparius and Allogamus sp. was not affected, and as expected, specialists were more efficient than generalists in exploring leaves as a dietary resource. However, results indicated that these fungicides have direct negative effects on microbial decomposers, and thus may compromise ecosystem functions on the long term.