Fungicidal effect of 'Biolide' disinfectant

Abstract

Conditionally pathogenic and pathogenic microflora negatively affects the general condition and productivity of animals, even with the provision of proper feeding and maintenance conditions. Significant damage to industrial farms is caused by fungal infections such as Aspergillus, Penicillium, Fusarium, and Candida albicans which associate with using of different disinfectants. The work aimed to determine the effective concentration of “Biolide” for disinfection concerning reference strains Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404. The tests were conducted in the State Scientific Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise. We used bactericidal drug “Biolide” based on hydrogen peroxide, perlactic acid and lactic acid. We determined the fungicidal action of different concentrations of “Biolide” and parameters of use following generally accepted recommendations. The study and determination of fungicidal concentrations of “Biolide” were carried out by the following methods: suspension, paper disks. For this purpose, we prepared the following aqueous solutions of “Biolide” — 0.5, 1.0, 2.0, 2.5% and prepared spore suspensions of reference strains of Candida albicans ATCC 10231 (2.5×107/см3) and Aspergillus niger АТСС 16404 (1.8×107/см3). Control: the culture of fungi with working dilutions was the control. The tests revealed that a 2%-concentrated “Biolide” disinfectant has fungicidal effect concerning the reference strains Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404. “Biolide” disinfectant solution at 2.0% concentration and an exposure time of 60 minutes exhibits fungicidal properties on the test objects (wood, iron, brick, plaster)