Abstract

water was applied and the prothallia floated off into a bowl. A spoon was used in transferring the prothallia to pots of moist peat. In each spoonful of the liquid there were numbers of prothallia, barely large enough to see without the aid of a glass, yet large enough to permit a fairly even distribution. The pots were covered with glass jars. The above formula is a very good one for growing fern spores on agar, but more than ordinary care is required because an aseptic condition must be maintained. The results obtained are not worth the extra trouble and expense involved. The flower pot and rock method will not compare with peat, which in my judgment is not only the most practicable, but affords the maximum protection. WASHINGTON, D. C.

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