Abstract

This study was focused on detection of the spectrum of fungi in the wood of stone fruits using molecular genetic methods. Samples were obtained from apricots, plums and sweet cherry trees from region of Moravia, one sample was obtained from Myjava (Slovakia). Segments of symptomatic wood were obtained from dying stone fruit trees with very significant symptoms. This study describes detection of the fungi in the wood of 11 trees in general in 5 localities. The cultivation of the fungi from symptomatic wood and sequencing of ITS was carried out. Eleven fungal genera were determined in the stone fruits wood, particularly Irpex lacteus, Fomes fomentarius, Neofabraea corticola, Calosphaeria pulchella, Cytospora leucostoma, Phellinus tuberculosus, Stereum hirsutum, Collophora sp., Pithomyces chartarum, Aureobasidium pullulans,Fusarium sp. The results of this study demonstrate that the reason of declining of stone fruit trees in Moravia is caused probably by trunk pathogens.

Highlights

  • Diseases of fruit trees have a negative effect on their yield and viability in growing regions around the world (Gramaje et al 2010, 2012; Bertsch et al 2013)

  • trunk disease (TD) fungal species are complex of taxonomically unrelated fungi from Botryosphaeriaceae and Diatrypaceae family and species of genera Cadophora, Campylocarpon, Collophora, Cylindrocarpon, Cylindrocladiella, Dactylonectria, Ilyonectria, Phaeoacremonium and Phaeomoniella and fungi of Basidiomycetes

  • Nowadays stone fruit growers leave this activity in the Czech Republic

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Summary

Introduction

Diseases of fruit trees have a negative effect on their yield and viability in growing regions around the world (Gramaje et al 2010, 2012; Bertsch et al 2013). Syringae) or still world spreading Xylella fastidiosa, a phytoplasma 'Candidatus Phytoplasma prunorum' or, 'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma pyri' and last but not least fungal trunk disease (TD) pathogens. The history of fruit growing in the Czech Republic has a long tradition. Stone fruits grown traditionally in the Czech Republic include the plums, the cherries, and in the warmer areas the apricots. From 1950 till stone fruit production areas in the Czech Republic are decreased rather and/or stagnate. Nowadays stone fruit growers leave this activity in the Czech Republic

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