Fungal toxins affect the fitness and stable isotope fractionation of Collembola

Abstract

We investigated the effect of the fungal toxin sterigmatocystin on the fitness and stable isotope fractionation of two Collembola species (Folsomia candida and Heteromurus nitidus) feeding on mixed vs. single diets. Four knock out mutants of Aspergillus nidulans with the sterigmatocystin production blocked at different steps along the biosynthetic pathway were combined in mixed diets with either the high quality fungus Cladosporium cladosporioides or the low quality fungus A. nidulans (wildtype). Using fungi labeled with stable isotopes (13C and 15N) we evaluated the incorporation of carbon and nitrogen from individual fungi. We hypothesised that (i) Collembola fitness decreases with the putative toxicity of the fungi (ii) Collembola benefit from ingestion of mixed diets due to toxin dilution and (iii) fractionation of 13C and 15N is more pronounced in more toxic diets. Mixed diets did not uniformly improve fitness. Toxin dilution, however, played an important role in Collembola fitness. The fractionation of 13C and 15N varied with sterigmatocystin mutant strains, and Collembola species often differed from the expected enrichment per trophic level. The results show that fungal toxin production may affect stable isotope fractionation, presumably by altering consumer excretion rates necessary for detoxification.