Abstract
ABSTRACTSecond-generation bioethanol derived from native perennial grasses offers a promising alternative for biofuel, especially when the biomass avoids land-use competition for crop production. Panicum prionitis Ness is a native perennial C4 grass predominant in soils of the Delta del Paraná River, Argentina. Its forage quality (palatability and digestibility) for livestock is low because of its substantial lignin content. In this work, we evaluated different pretreatments (phosphoric acid, ligninolytic enzymes and fungal secretomes) aimed to degrade lignin and improve cellulose hydrolysis efficiency. Results show that 2-day pretreatments with fungal secretomes highly improve release of fermentable sugars compared with conventional pretreatments. Although Pycnoporus sanguineus displayed a greater contribution than Ganoderma applanatum to the pretreatment, the latter triggered the highest final yield, achieving a hydrolysis of 47.5% of cellulose when added to green tissue. These results strengthen the feasibility of using Panicum prionitis biomass in a low-polluting bioethanol production process.
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