Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels.

Abstract

Fungal laccase-mediated destaining of Coomassie Brilliant Blue-R-250 stained polyacrylamide electrophoretic gel is very sensitive and environment friendly. Fermenting of Cerrena sp. HYB07 yields a broth with laccase activity which when supplemented with distilled water and used as a destaining solution results in a clear gel background in 2h at 37°C. Also, separation of the laccase enzyme for efficient functioning is not required. The method utilizes gel destaining and process of decolorizing dye simultaneously with a detection sensitivity of up to 10 ng. This technique avoids the use of hazardous organic compounds, as the destaining solution and the destain solution could be discarded easily without any further treatment.