Abstract
Brewery sludges, consisting of spent grains and spent grain liquor, were used as fermentation substrates in aerobic batch cultures of Aspergillus niger. The results showed that the fungus was able to utilise the wastes, producing certain amylolytic and cellulolytic enzymes (amylase, EC 3.2.1.1; amyloglucosidase, EC 3.2.1.2; cellulase, EC 3.2.1.4), and increased biomass, during the fermentation process. Optimum fermentation temperature and pH were about 29°C and 6·1, respectively. Enzyme concentration was correlated with growth of the fungus, and it was also dependent on the type of nitrogen and phosphorus salts in the medium. The spent grains were a better substrate than the spent grain liquor. Diets based on the fermented grains were well accepted by broiler chickens. They elicited better growth than control diets made from non-fermented spent grains.
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