Abstract
In present study, statistical optimization of medium composition for endoinulinase production from raw inulin using Aspergillus tritici was performed in shake-flask fermentations. The concentration of four independent variables viz. raw inulin (1.5–3.5%), peptone (0.2–1.8%), (NH4)H2PO4 (0.2–1.8%) and pH (4.5–6.5) was explored to optimize the composition of fermentation medium for maximum endoinulinase production. Fermentation medium containing 2.5% raw inulin, 1% peptone, 1% (NH4)H2PO4 and medium pH adjusted to 5.5 supported maximum (25.39 IU/mL) endoinulinase production. Adequate precision value >4, closeness of coefficient of determination (R2) value to 1, good co-relation between the predicted and experimental values, percentage error <5%, high F-value 164.52 and low Lack of fit F-value 0.19 authenticates the fitness of quadratic model. TLC fingerprints studies confirmed fructooligosaccharides synthesis from inulin by crude fungal endoinulinase. Fructooligosaccharides yield (19.40%) containing 3.70% ketose (GF2), 2.71% nystose (GF3) and 1.42% fructofuranosyl nystose (GF4) were obtained from inulin (10%).
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