Abstract
Fungal concentrations in dust samples analyzed by molecular methods were carried out of animal rearing house at Hessaraghatta village, Bangalore. Handy air sampler EnviroTech-APM 821 was used to collect the dust samples in triplicate and sampling time was limited to eight hours. The sampled filter papers were used for the assay of DNA extracted from all the four animal rearing locations were used for PCR amplification using Universal ITS fungal identification primers PCR products were analyzed on 2% agarose gel out of four different locations were rabbit house, cow shed, poultry farm and swine house. The sequencing was then blasted against known sequences in the NCBI database to provide species identification and phylogenetic tree was also constructed with 20 closely related blast sequences. The rabbit house and cow shed dust samples did not show any amplifications even with varying conditions while poultry farm (Cladosporium oxysporium DFFSCS018) and swine house (Peniophora species1306-hpF-04) isolate samples gave amplicons of 550bp and 600bp respectively with ITS region showing 99% similarity. These studies useful for clinicians to identify the cause of the dust allergic diseases of the animal rearing house workers and suggest amendment strategies.
Highlights
Recent rapid advances in molecular biology provide the potential for enhanced characterization and rapid quantification of microorganisms in the environment
The sampling site selected for fungal concentrations in dust samples of animal rearing houses were analyzed by Polymerase Chain Reaction (PCR) methods were Location 1 - Rabbit house, Location 2 Cow shed, Location 3 - Poultry farm and Location 4 Swine house at Hessaraghatta village, Bangalore in the State of Karnataka, India
DNA was isolated from the dust samples of four different animal rearing site and all the DNA samples were analyzed on 0.8% agarose gel and visualized under UV gel Documentation unit (Fig. 1)
Summary
Recent rapid advances in molecular biology provide the potential for enhanced characterization and rapid quantification of microorganisms in the environment. It is used to copy and amplify many million-fold specific regions (typically
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
