Fundamental Analytical Parameters of a Glucose Biosensor Based on TiO2 Nanotube Arrays and Chitosan as Immobilization Matrix

Abstract

first_page settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Fundamental Analytical Parameters of a Glucose Biosensor Based on TiO2 Nanotube Arrays and Chitosan as Immobilization Matrix † by Margalida Artigues-Cladera, Jordi Abella and Sergi Colominas * Electrochemical Methods Laboratory, Department of Analytical and Applied Chemistry, ETS Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain * Author to whom correspondence should be addressed. † Presented at the 5th International Symposium on Sensor Science (I3S 2017), Barcelona, Spain, 27–29 September 2017. Proceedings 2017, 1(8), 832; https://doi.org/10.3390/proceedings1080832 Published: 20 November 2017 (This article belongs to the Proceedings of Proceedings of the 5th International Symposium on Sensor Science (I3S 2017)) Download Download PDF Download PDF with Cover Download XML Versions Notes Numerous analytical techniques are used for glucose determination, such as spectrophotometry, amperometry, HPLC, polarimetry and capillary electrophoresis. However, only electrochemical biosensors based on the use of glucose oxidase (GOx) have been able to combine the analytical power of electrochemical techniques with the specificity of biological recognition processes. Commonly, this combination of biological components with electrodes is used to produce low-cost, easy-to-use and compact devices for glucose quantification. Since the development of the first glucose biosensor, great efforts have been made in order to improve the response performances of these enzyme electrodes.In the present work, we present the fundamental analytical parameters of an amperometric glucose biosensor based on GOx immobilization using a polymeric hydrogel (Chitosan) onto highly ordered titanium dioxide nanotube arrays (TiO2NTAs). The biosensor optimal working potential was evaluated and then fixed at −0.4 V. After that, the fundamental analytical parameters of the biosensor (linear range, limit of detection, sensitivity) were determined, as well as its storage stability. This biosensor showed a linear range from 0.3 mM to 1.5 mM, low limit of detection (0.07 mM) and high sensitivity (5.46 mA·mM−1). Furthermore, its lifetime was evaluated. After 30 days, the biosensor retained 85% of its initial current response. © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Artigues-Cladera, M.; Abella, J.; Colominas, S. Fundamental Analytical Parameters of a Glucose Biosensor Based on TiO2 Nanotube Arrays and Chitosan as Immobilization Matrix. Proceedings 2017, 1, 832. https://doi.org/10.3390/proceedings1080832 AMA Style Artigues-Cladera M, Abella J, Colominas S. Fundamental Analytical Parameters of a Glucose Biosensor Based on TiO2 Nanotube Arrays and Chitosan as Immobilization Matrix. Proceedings. 2017; 1(8):832. https://doi.org/10.3390/proceedings1080832 Chicago/Turabian Style Artigues-Cladera, Margalida, Jordi Abella, and Sergi Colominas. 2017. "Fundamental Analytical Parameters of a Glucose Biosensor Based on TiO2 Nanotube Arrays and Chitosan as Immobilization Matrix" Proceedings 1, no. 8: 832. https://doi.org/10.3390/proceedings1080832 Find Other Styles Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here. Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.