Abstract
We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM.
Highlights
The scope of H2O2 is very broad in our days, it affects many areas like chemistry [1], food industries [2], clinical applications, and environmental chemistry [3]
The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface
The detection of hydrogen peroxide was successfully observed in phosphate buffered saline (PBS) for both biosensors using the cyclic voltammetry and the chronoamperometry techniques
Summary
The scope of H2O2 is very broad in our days, it affects many areas like chemistry [1], food industries [2], clinical applications, and environmental chemistry [3]. The electrochemical methods are very selective, sensitive, and illustrate low detection limits for hydrogen peroxide [2, 8, 11]. Palladium nanoparticles with small size (1 nm) were used due to their higher electron conductivity In this present work, two biosensors were developed for hydrogen peroxide detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with small palladium nanoparticles
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