Abstract
Multilayered nanofilms, functionalized with growth factors, were developed to act as a reparative scaffold, stimulating cell proliferation, differentiation and tissue formation in a skin wound model. Poly L‐Lysine and Poly L‐Glutamic Acid, incorporating platelet derived growth factor and transforming growth factor‐β, were electrosprayed onto a wounded skin equivalentas a ten‐bilayer nanocoating and cultured for a 6‐day period. Control cultures received an applied nanocoating with no growth factors. Zeta potential (ZP) & quartz crystal microbalance (QCM) data were used to characterize film construction. Histochemical (HC) and immunohistochemistry (IHC) analyses were performed to assess cell proliferation and tissue formation. ZP and QCM data indicated that the nanocoatings were deposited evenly & maintained a charge differential between layers. HC and IHC results show that proliferation was highest in epidermal vs. dermal layers and IHC studies suggest that histogenesis was greater in the dermis. Control cultures showed a very poor wound response. The quality of tissue regenerated will determine the large‐scale scope of this therapy but preliminary results suggest a potential new treatment modality. Funding provided by the State of Louisiana Governor's Biotechnology Initiative.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.