Abstract
Legionella pneumophila is a pathogenic bacterium that proliferates in a large variety of environmental water sources. It can cause a severe infection, legionellosis, resulting in a fatal form of pneumonia. Therefore, developing detection platforms such as highly specific and sensitive biosensors is of great importance. Surface Plasmon Resonance imaging (SPRi) has become one of the most widely used label-free method for fast and sensitive detection of chemical and biological analytes on sensor surfaces. We propose here a biosensing approach based on DNA capture and detection probes that specifically target the 16s rRNA sequence of pathogenic L. pneumophila, using Surface Plasmon Resonance imaging technique and gold nanoparticles for signal enhancement.
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