Abstract
Early screening is an important way to reduce the incidence and mortality of colorectal cancer (CRC). Fecal DNA testing stands out among many screening methods due to its high sensitivity. However, at this stage, researchers have not found a high-efficiency method for fecal DNA extraction. To this end, this work carried out a new round of exploration. Here, this experiment synthesized a kind of nanomagnetic beads (NH2-SiO2@Fe3O4) with good stability for nucleic acid extraction. A comparative study with the centrifugal adsorption column method revealed the significant advantages of the magnetic bead method in extracting fecal DNA. The DNA extracted by the magnetic bead method is of high purity, can also achieve high-throughput tests, and is more suitable for polymerase chain reaction detection, greatly simplifying the stool DNA detection process and providing a basis for the widespread promotion of early screening.
Highlights
Colorectal cancer (CRC) has become the third most malignant tumor in the world [1,2,3,4]
Most CRCs develop from normal mucosa to colorectal adenomas, and this malignant transformation generally takes a long period of time [5,6,7,8]
3.2. e Effect of Extraction Methods on Fecal DNA Purity. is experiment has studied the difference in UV absorption of different nucleic acid extraction methods, and the results are shown in Figure 2. e data show that the UV absorption spectrum of stool DNA extracted with NH2-SiO2@Fe3O4 nanomagnetic beads has obvious peaks and valleys (Figure 2(a)), while the UV absorption spectrum of stool DNA extracted by the spin column method has no obvious peaks and valleys (Figure 2(b))
Summary
Colorectal cancer (CRC) has become the third most malignant tumor in the world [1,2,3,4]. Statistics in 2018 show that the incidence of CRC is about 10.2%, and the mortality rate is about 9.2%. From this point of view, the health threat CRC poses to the world’s population cannot be underestimated. Most CRCs develop from normal mucosa to colorectal adenomas, and this malignant transformation generally takes a long period of time [5,6,7,8]. E survival rate of patients with early diagnosed colorectal cancer in the five years after surgery is much higher than that of patients with advanced CRC [9,10,11,12]. Researchers have made more efforts in the exploration of early cancer screening methods
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