Abstract
Carbon quantum dots (CQDs), in comparison to heavy metal-based quantum dots offer renewable, non-toxic, low cost and easy synthesis production route while having excellent physicochemical properties for biomedical or environmental use. This paper discusses development and application of functionalized carbon quantum dots from glycerol as primary carbon source and tetraethylene pentamine as functionalizing agent. As-synthesized CQDs were characterized by Fourier Transform Infrared Spectrometer (FTIR), UV-Vis Spectrophotometer and Spectrofluorometer. FTIR spectra confirmed functionalization of resulting CQDs with emission wavelength peaks at 314.18 nm and 381.15 nm and observed strong blue luminescence under UV lamp. The performance of produced CQDs as bio-fluorophore for Gram-stained bacterial models was validated. Results indicated that CQDs can effect fluoresced images but a more distinct image can be observed on S. aureus compared to E. coli. An attachment mechanism of carbon quantum dots to the bacteria surfaces was also proposed here.
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